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In trauteine infection was known to be related with preterm labor. Thes study was designed to evaluate the effect of E.coli endotoxin on production and metabolism of prostagrandin E2(PGE2), of which the important role in human parturition is widely accepted, by dispersed cells from human amnion. The amnion cells were established in primary monolayer culture from 5 normal term pregnant women undergoing elective cesarean sections between 38th and 40th weeks of gestation. Bacterial endotoxin were added in concentration of 10 ng/ml, 100ng/ml, 1000ng/ml to amnion cell suspensions, and the supernatant of incubation medias were obtained after incubation of 4 and 24 hours. The concentration of PGE2 and 11-deoxy-13, 14-dihydro-15-keto-11B, 16e-cyclo PGE2 ( bicyclo-PGEM) released in all bacterial endotoxin concentrations at both 4 and 24 hours of incubation. These data suggest bacterial endotosin and probably intrauterine infection contributes to preterm labor by PG production.
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